HoloMonitorM4
M4激光全息細(xì)胞成像分析系統(tǒng)，配備和M3同樣強(qiáng)大的細(xì)胞圖像分析軟件，實(shí)現(xiàn)在細(xì)胞正常生
長(zhǎng)環(huán)境下的非侵入，非標(biāo)記活細(xì)胞成像及分析。
M4儀器非常精巧，可以放置在培養(yǎng)箱中，實(shí)現(xiàn)非常wan美的Timelapse（延時(shí)成像）分析。同時(shí)，
測(cè)量并分析細(xì)胞成像，細(xì)胞計(jì)數(shù)，細(xì)胞面積和體積等形態(tài)學(xué)參數(shù)。

M4性能優(yōu)勢(shì)
*實(shí)時(shí)3D成像
*直接測(cè)量貼壁細(xì)胞
*非標(biāo)記，無需染色，無光毒性
*細(xì)胞面積和體積分析
*長(zhǎng)期time-lapse成像分析
*細(xì)胞跟蹤功能
*體積小巧，可直接放在培養(yǎng)箱中

下面一組Time-lapse圖片展示了人巨噬細(xì)胞吞噬酵母細(xì)胞時(shí)形態(tài)的改變

如下一組Time-lapse圖片展示了人MCF-10A乳腺細(xì)胞（30分鐘間隔拍攝）

技術(shù)參數(shù)：
圖像類型:定量相位圖像
CCD相機(jī):1280×1024像素,8or10bits(全息圖)
光源:單色激光(全息模式)
物鏡:20x全息
計(jì)算機(jī):處理器IntelCorei7,Memory6GB,Harddrive1TB,GraphicsNVidia
1GB,Screen1920x1080,FullHD,Windows7Professional
軟件:Holostudio2.3圖像和細(xì)胞分析軟件

系統(tǒng)性能
工作距離:1.2mm
數(shù)字聚焦范圍:最大50×景深
抓拍時(shí)間:低于4μs/圖像
立體取樣:1024×1024pixels(全息圖)
捕獲速度:4fps(1024×1024像素)
重建速度:4fps(512×512像素),1fps(1024×1024像素)
樣品照度:~0.1mW/cm
樣品容器高度:最大45mm
電壓:100-249VAC-47/63Hz功率(w/oPC):max.80W
體積(L×W×H):250×160×180mm重量:2.5kg

自2008年起，瑞典Phiab公司的激光全息分析系統(tǒng)就有大量用戶文獻(xiàn)發(fā)表，特挑選近三年文獻(xiàn)與大家分享。

2014

Live-cell mass profi領(lǐng): an emerging approach in quantitative biophysicsNature Methods

The role of pi3k/akt-related pip5k1α and the discovery of its selective inhibitor for treatment of advanced prostate cancerPNAS

Quantitative stain-free and continuous multimodal monitoring of wound hea領(lǐng) in vitro with digital holographic microscopyPLoS ONE

Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in l929 cellsPLoS ONE

Edaravone protects against methylglyoxal-induced barrier damage in human brain endothelial cellsPLoS ONE

Environmental and toxicological impacts of glyphosate with its formulating adjuvantInternational Journal of Agricultural, Biosystems Science and Engineering

Prevention and treatment of colon cancer by peroral administration of hamletGut

2015

From immobilized cells to motile cells on a bed-of-nails: effects of vertical nanowire array density on cell behaviorScientific Reports 2015

Enhanced cytotoxicity of folic acid-targeted liposomes co-loaded with c6 ceramide and doxorubicin: in vitro evaluation on hela, a2780-adr and h69-arMolecular Pharmaceutics

Targetedtumor therapy byrubia tinctorum l.: analytical characterization ofhydroxyanthraquinones andinvestigation oftheir selectiveCancer Cell International

Cathelicidins positively regulate pancreatic β-cell functionsThe FASEB Journal（江南大學(xué)發(fā)表）

Macrophage polarization and the effect of microrna-155 administered in water-in-oil-in-water multiple emulsion formulationsJournal of Clinical & Cellular Immunology

Oloxamer bioadhesive hydrogel for buccal drug delivery: cytotoxicity and trans-epithelial permeability evaluations using tr146 human buccalInternational Journal of Pharmaceutics

Incubator proof miniaturized holomonitor to in situ monitor cancer cells exposed to green tea polyphenol and preosteoblast cells adheringJ. Biomed. Opt

Reversal of chemoresistance in ovarian cancer by co-delivery of mdr inhibitors and paclitaxel using a targeted liposomal platformThe FASEB Journal

Induction of morphological changes in death-induced cancer cells monitored by holographic microscopyJournal of Structural Biology

2016

Shielding of the geomagnetic field alters actin assembly and inhibits cell motility in human neuroblastoma cellsScientific Reports (中科院生物物理所發(fā)表）

Pseudomonas aeruginosa n-3-oxo-dodecanoyl-homoserine lactone elicits changes in cell volume, morphology, and aqp9 characteristics inFrontiers in Cellular and Infection Microbiology

The curcumin analog c-150, influencing nf-κb, upr and akt/notch pathways has potent anticancer activityin vitroandin vivoPLOS ONE